Introduction to peripheral blood smear examination clinical. Ppt peripheral blood smear powerpoint presentation. Alternately, edta anticoagulated blood may be used if the smear is made within one. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. How to build a hinged hoophouse for a raised bed garden duration. Glass slides should be free of chipped edges, glass fragments, dust, powder, fingerprints, and other debris, as these can affect blood spreading. Blood films are examined in the investigation of hematological blood disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. Mar 05, 20 blood samples and preparation of smears. To this end, this video describes in a virtual way the realization of a blood smear and the staining of its cellular components with giemsa staining. Blood smears can be made from the blood remaining in the needle by pushing a small drop of blood onto the slide with the last evacuated tube.
A blood smear seeks to check out for abnormalities in the three main blood cells that are red cells, white cells and platelets. An extensive study was undertaken to compare five methods of blood smear preparation. The slide is allowed to airdry and is then stained specimen edta anticoagulated blood is preferred. Spreading of blood smear is very important step that precede many laboratory investigation like diagnosis of blood parasites, performing. In some ways it is becoming a lost art but it often provides rapid, reliable access to information about a variety of hematologic disorders. A blood smear is a blood test used to look for abnormalities in blood cells. Thick and thin blood smears were also prepared using the conventional method on glass slides. C m ac m a blood smear preparation materials and supplies sample edta slides fixative, buffer and stain diffquik water 4. Examination of thin blood films is important in the investigation and management of anaemia, infections, and other conditions which produce. The gardening channel with james prigioni recommended for you. Blood smear medical tests ucsf benioff childrens hospital. Ppt malaria blood smear powerpoint presentation free. Clean, highquality glass microscope slides are essential to blood smear preparation.
Mar 29, 2019 how to determine sex from a blood smear. Find out from the quiz what you need to know about peripheral blood smear. Three mechanical devices for preparing blood smears were compared to the traditional coverslip and manual wedge methods. There are multiple methods to blood smear preparation. The study of blood smear as the analysis of images of various objects. The staining was conducted using giemsa staining for the thick and thin smears. Seventyfour blood samples, from 35 women and 39 men, were haphazardly selected from the thousands of tubes submitted to us for complete blood counts. Assign to person add to training plan add to bookmarks bookmarking is a premium feature. Pdf the study of blood smear as the analysis of images of various.
Blood smears are used to determine leukocyte differentials, to evaluate erythrocyte, platelet and leukocyte morphology, and, if necessary, to estimate platelet and leukocyte counts. Blood smear synonyms, blood smear pronunciation, blood smear translation, english dictionary definition of blood smear. Neel, dvm, dacvp clinical associate professor, clinical pathology nc state college of veterinary medicine raleigh, nc, 27607 introduction although tremendous advances have been made in the field of pointofcare hematology analyzers, examination of a well prepared, well stained blood smear remains the. A blood smear is made from a drop of blood on a glass slide and is a useful diagnostic tool. Download this app from microsoft store for windows 10. It may be used to help diagnose andor monitor numerous conditions that affect blood cell populations. The blood film occupies the central portion of the slide and has definite margins on all sides that are accessible to examination by oil immersion. Cbc peripheral how the test is performed a blood sample is needed. Blood smear interpretation component test codes cannot be used to order tests. Dec 30, 2019 i think and im sure, for practicing it that doing an ihc on a blood smear is not a good idea. Because the red blood cell is full of endogenous peroxidases and other parasitic enzymes that are. Comparison of five methods for preparing blood smears. Choose from 500 different sets of preparing staining a blood smear flashcards on quizlet.
Peripheral blood smear procedure and interpretation. Megan brashear, cvt, vts ecc, demonstrates how to make a blood smear. The blood is streaked in a thin film over the slide. When first learning, try multiple methods to determine which process works best for you. These are the same cases that you studied in the two labs the normal smear is in there as well. At one time, a blood smear was prepared on nearly everyone who had a cbc. The phrase blood smear is descriptive of how the preparation is made. One can make smears not only of peripheral blood circulating blood, taken from a vein but also of bone marrow where blood is formed or of inflammatory infiltrate pus. Introduction to peripheral blood smear examination oncohema key. Blood smear definition of blood smear by medical dictionary.
All of these samples belonged to patients hospitalized in our establishment regardless of their illness or their treatment. When a peripheral blood sample is smeared on a slide and stained, it is known as a. Blood smear health encyclopedia university of rochester. Examination of a blood smear is an integral part of a hemogram. Malaria blood smear remains the gold standard for diagnosis giemsa stain distinguishes between species and life cycle stages parasitemia is quantifiable a free powerpoint ppt presentation displayed as a flash slide show on id. A blood smear is often used as a followup test to abnormal results on a total blood count cbc to evaluate the various types of blood cells. Smears made from edtaanticoagulated blood should be made. Get free instant downloader for youtube microsoft store. A drop of blood is placed on the slide, smeared across it, fixed, dried, stained, and covered. It is often done as part of or along with a complete blood count cbc. Jun 12, 2016 1 introduction to peripheral blood smear examination. Peripheral smear preparation staining of peripheral blood smear peripheral smear examination objectives 6. Ppt preparation, staining and examination of blood film.
Preparation, staining and examination of blood film 1 preparation, staining and examination of blood film. This is making a blood smear by dkit vet nursing on vimeo, the home for high quality videos and the people who love them. Slides should not be left uncovered on top of the counter. Learn preparing staining a blood smear with free interactive flashcards. If the slide has been stored in spirit, ensure that all the spirit has been thoroughly washed away with water and the slide well dried with a soft, lint free cloth or paper tissues. C m ac m a a drop of blood is placed on a clean microscope slide. Traditionally, trained laboratorians have examined blood smears manually using a microscope. Notice the angle and direction that the spreader slide is pulled, first back towards. The purpose of preparation of blood smear blood film is to study the morphology of rbcs, differential leukocyte count and reticulocyte count. Aug 30, 2010 a blood smear gives information about the number and shape of blood cells.
Present blood smear to your instructor for evaluation. Clean slides with 70 to 90% alcohol and allow to dry. The other is pusher or the spreader slide drop of blood 23 mm in diameter. Place a drop of blood, about 23 mm in diameter approximately 1 cm from one end of slide.
The three main blood cells that the test focuses on are. Smears from opaque thick liquids or semisolids, such as stool, can be made using a swab to sample and smear the material. Blood smears peripheral blood smear is a very important tool in the hematology lab it provides rapid, reliable access to information about a variety of hematologic disorders examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults the smear offers a window into the. Steps to prepare blood and new methylene blue to make smear. A properly performed and analyzed blood smear is the most informative of all hematologic tests, allowing examination of erythrocytes. Making and staining a blood smear university of vermont. The smear shows the number and kinds of white blood cells differential, abnormally shaped blood cells, and gives a rough estimate of white blood cell and platelet counts. Each person may have their own preferencemethods for success. C m ac m a a spreader slide is used to feather the drop of blood. Blood smears are made using fresh blood or edta blood.
It might be used to help identify andor keep an eye on numerous conditions that impact blood cell populations. Leishmans stain maygrunwaldgiemsa stain jenners stain wrights stain principle. Two easy methods for blood film preparation and staining youtube. Blood smear definition of blood smear by the free dictionary. Label precleaned slides preferably frostedend with patients name or other identifier, date and time of collection.
A properly prepared blood smear is essential to accurate assessment of cellular morphology. Purpose and criteria for blood smear scan, blood smear. A blood smear is a blood test that gives information about the number and shape of blood cells. The blood can be obtained from finger heal microhematochrit tubes non heparinised for edta or heparinised for capillary blood wooden stick or glass capillary pipette.
The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. Making and staining a blood smear a wellmade blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. Preparation of peripheral blood smears exercise 10. Nov 30, 2014 blood smears peripheral blood smear is a very important tool in the hematology lab it provides rapid, reliable access to information about a variety of hematologic disorders examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults the smear offers a window into the. Find a good area of the slide then scan at 25x or 50x examining cells at 100x as needed. A blood smear is used to diagnose andor monitor numerous conditions including appropriate number and morphology of white blood cells wbcs, red blood cells rbcs, andor platelets plt. There, the lab technician looks at it under a microscope. Peripheral blood smear examination macroscopic view. Video tutorial on how to perform a proper thick and thin smear for malaria diagnosis by stephane proux, malaria diagnosis lab supervisor at. Blood smear preparation and staining 1 blood smear preparation and staining.
Reticulocytes, young rbcs and inclusion bodies on cells. Microscopic examination 1,000x of the thin and thick blood smears prepared on transparent acetate produced highquality images for both the parasites and the blood cells. Blood smear preparation pdf download 3d39b66ab9 this material enables the simple and lowcost preparation of samples and does not compromise the morphologic and staining characteristics of the parasites or blood cellsthis ensured that the entire length of the smear on the acetate was simultaneously stainedor its licensors or contributorssecond. Standardization of blood smears prepared in transparent. The smear is greater than 25 mm long and the feathered edge stops approximately 10 mm from the end of the slide. Blood smear preparation properly prepared blood smears are essential to accurately assess the patients status. A free powerpoint ppt presentation displayed as a flash slide show on id. A blood filmor peripheral blood smear is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. The new protocol can be used to stain blood smears, bone marrow slides, and slides of other bodily fluids. Feasibility of counting smudge cells as lymphocytes in differential. When the needle is inserted to draw blood, some people feel moderate pain.
Hi, megan, a couple of comments it would be beneficial if you showed a closeup of the finished slide so that viewers could appreciate what you describe. Peripheral blood smear dr ajit nambiar spherocytes causes hereditary spherocytosis immune hemolytic anemia severe burns invitro prolonged storage of blood. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. A blood smear is a drop of blood spread thinly onto a glass slide that is then treated with a special stain and the blood cells on the slide are examined and evaluated. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Download 1776 blood smear stock photos for free or amazingly low rates. Principle of smear preparation smear preparation a small drop of blood is placed near the frosted end of a clean glass slide. To make a good blood smear, very little equipment is needed aside from practice in how to create a good smear and a good microscope. Take another clean slide, and holding at an angle of about 45 deg, touch the blood with.
The blood film should cover about three quarters of the length of the slide. Blood smear with interpretation arup lab test directory. Blood smear stock photos download 1,776 royalty free photos. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Two easy methods for blood film preparation and staining duration.
Push the spreader slide forward in a rapid, steady, even motion to produce a blood film that is thick at one end and that tapers to a thin, feathered edge at the other. This is a blood test to look at the number and shape of your red and white blood cells and platelets to see whether they are normal. Proper blood smear preparation texas veterinary medical. A variety of methods are available for preparing and staining blood smears, the most common of which are discussed in this atlas. A blood smear is often used as a followup test to abnormal results on a complete blood count cbc to evaluate the different types of blood cells. Introduction to peripheral blood smear examination. Jan 09, 2017 a blood smear is often used as a followup test to abnormal results on a total blood count cbc to evaluate the various types of blood cells. A series of five slides was prepared from each sample of blood. Dec 08, 2006 a well prepared blood smear is necessary for microscopic examination of blood.
Or, the blood may be examined by an automated machine. To perform a blood smear, two very clean slides are re. The information provided here is not sufficient for interface builds. Feb 27, 2017 proper technique for blood smear preparation. Prepare a blood smear according to the instructions. Preparation of blood smears in order to reduce the number of unacceptable smears received, marshfield labs recommends the following procedure to be used in preparation of blood smears. Sysmex spi for automated bone marrow slide smear staining. This picture illustrates the proper procedure for making blood smears. Peripheral blood or potassium edta anticoagulated blood 12 mg edta1 ml blood may be used. Peripheral blood smear, blood smear analysis, peripheral blood film, smear. Blood smear analysis allows quantitation of the different types of leukocytes called the differential count, estimation of the platelet count, and detection of morphologic abnormalities that may be indicators of pathophysiologic processes.
Blood smear preparation and staining blood smear preparation and staining prepared by. Examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Blood smear article about blood smear by the free dictionary.